All patients were treated for a minimum of 12 weeks. IGF-binding protein-3 (IGFBP-3) was measured using a specific RIA with an intra-assay variability of 7.3% at 2772 ng/ml and 6.9% at 3545 ng/ml [19]. After completion of the hemodynamic measurements blood was drawn to determine plasma adrenaline and noradrenaline by high-performance liquid chromatography (Chromosystems, Munich, Germany). et al.Â, Tsutamoto Right heart catheterization was performed in the fasting state without the morning medication between 9.00 and 11.00 h. After positioning the catheter in the pulmonary artery the patient was brought to a quiet room where measurements were taken after 30, 90 and 150 min. We thank the participating patients and all those who helped with the running of the study, particularly Ingrid Jackwerth, Anke Heiser, Elke Szczech, Matthias Friedrich, Udo Kuhnert, and Rainer Stein. Goodfellow Nitric oxide (NO) is a mediator of vasodilation in blood vessels. Bode-Böger 193:321–328. Wert D.V. 1). Freudenberg-Plessow Urinary excretion rates of nitrate and cyclic GMP were corrected by urinary creatinine concentration to limit variability due to differences in renal function [17]. Imaizumi [13] were the first to demonstrate that the vasodilator effect of IGF-I in the isolated perfused rat kidney is abrogated by the NO-synthase inhibitor l-NMMA. International Journal of Molecular Medicine, 45, 1195-1202. https://doi.org/10.3892/ijmm.2020.4476. Endothelium-dependent vasodilation is impaired in patients with chronic heart failure [1]. 1, Table 2). There is evidence for reduced activity of the GH-IGF-I axis in patients with heart failure [8]. 123:86–92. Int J Mol Med. Kästner This study was supported in part by grants from the Dr.-Karl-Wilder Stiftung, Bonn, Germany and from the Max-Delbrück-Center for Molecular Medicine, Berlin, Germany. Abstract. H. View Article : Google Scholar : PubMed/NCBI, Smith PK, Krohn RI, Hermanson GT, Mallia methodology to clinical perspectives. et al.Â. Serum levels of GH, IGF-I and IGFBP-3 were determined at rest in the morning of two subsequent days at baseline and after readmission and the average of both values was used for further analysis. R.H. U. Haass In the present study, we investigated whether systemic NO production is increased in patients with dilated cardiomyopathy during treatment with recombinant human growth hormone. Alterations of serum IGF-I, urinary nitrate and cyclic GMP excretion rates by GH or placebo treatment. kinase-mediates androgen receptor-dependent non-genomic activation 2014. 60:19–26. Overexpression of uncoupling protein 2 inhibits the high of signaling cascade leading to endothelial nitric oxide synthase. https://pubmed.ncbi.nlm.nih.gov/9799047/ Nitric Oxide: Study 1: Strohm C. Role of phosphatidylinositol 3-kinase/Akt pathway. S. A. of recombinant human GH dry powder which was mixed with 0.3% m-cresol solution upon insertion of the vial into the Kabi-pen (provided by Pharmacia & Upjohn, Erlangen, Germany). Gordienko Assessment of endothelium-independent vasodilation: From Microcirculation. J. There is evidence for reduced activity of the GH-IGF-I axis in patients with chronic heart failure [7,8].Treatment with GH for 3 months has been shown to increase left ventricular mass [9]. Hoegler Recruitment was stopped after randomisation of 50 patients. J M. Ramsey R.M. Pang 2013. K. R. D.R. Wu WZ, Hu DJ, Wang ZY, Liao LS and Li CC: Endothelial progenitor cell impairment mediated vasodilation dysfunction via diminishing nitric oxide production in postmenopausal females. Nitric oxide (NO), its metabolites and oxidation states are now recognized to play a significant role in the ability of red blood cells to control hypoxic vasodilation. Lorusso W. R. The study was approved by the Ethics Committee of the Max Delbrück Centre for Molecular Medicine, Berlin. Maier Gu L, Lian D, Zheng Y, Zhou W, Gu J and Liu X: Echinacoside‑induced nitric oxide production in endothelial cells: Roles of androgen receptor and the PI3K‑Akt pathway. In this review we focus on the potential role of nitric oxide in vasodilation of muscle resistance vessels during a bout of exercise. From: Endothelium and Cardiovascular Diseases, 2018. Cell Physiol Biochem. Growth hormone (GH) substitution therapy leads in GH-deficient patients to significant increases of IGF-I which may alter systemic vascular resistance by stimulating NO production. As activity of iNOS is not modulated via receptor-mediated mechanisms like IGF-I, NO formation by iNOS may have obscured the relation of NO synthesis and IGF-I in our study. Fulton et al. Co-factors for NOS include oxygen, NADPH, tetrahydrobiopterin and flavin adenine nucleotides. von der Leyen Baseline characteristics of the placebo and growth hormone treated (GH) groupa. Gu, L., Lian, D., Zheng, Y., Zhou, W., Gu, J., Liu, X. The changes from baseline (Δ Placebo and Δ GH) were compared and the respective P values were denoted in the last column. 2). Urine at baseline and after 3 months of therapy was obtained. LV ejection fraction was determined by a standard 1.0 Tesla whole-body imaging system (Siemens Expert Magnetom, Siemens AG, Erlangen, Germany) using an FISP (Fast Imaging with Steady State Precession) gradient echo sequence for gradient-echo-imaging (TR=RR−interval; flip angle 30°, echotime 13 ms). The reference ranges are 30–85 pg/ml and 185–275 pg/ml, respectively. androgen receptor trafficking. P. Strobel Nitric oxide relaxes the smooth muscle of the arterioles and produces vasodilation via both direct and indirect mechanisms. Hornig Privacy Policy. View Article : Google Scholar : PubMed/NCBI, Ahmad KA, Ze H, Chen J, Khan FU, Chen X, Vascul Pharmacol. J.C. Linz Verhovec Kübler et al.Â. Body weight and NYHA classification had not changed significantly in either of the groups after 98±1 days of therapy. et al.Â, Drexler GH in the evening. Related terms: Vasoconstrictors; Hypertension; Nitric Oxide; Eicosanoid Receptor The baseline clinical characteristics did not differ significantly between the patients assigned to placebo and GH with respect to height, weight, NYHA classification, ejection fraction, medication (Table 1), renal function, and baseline hemodynamic variables (Table 2). et al.Â, Fazio Drexler Oliveira LP, Costa RMD, Tostes RC, Georg RC, Costa EA, Lobato NS, 2002; 86: F41–F45. bisphenol A-induced testicular and sperm damage in rats through Blood pressure was determined automatically using a cuff (Siemens, Erlangen, Germany) and the average heart rate was taken from the ECG. Echinacoside and Cistanche tubulosa (Schenk) R. wight ameliorate Press; Beijing: pp. Serum creatinine did not change in both groups but there were significant increases of urinary nitrate and of cGMP excretion rates in the GH group (Fig. Y.Y. Riede View Article : Google Scholar : PubMed/NCBI, Maruhashi T, Kihara Y and Higashi Y: Impaired endothelium-dependent vasodilation leads to elevated peripheral vascular resistance which further increases left ventricular afterload at rest or during exercise [2,3]. C. Tsukahara View Article : Google Scholar, Yoshikawa M, Matsuda H, Morikawa T, Xie H, Bode-Böger View Article : Google Scholar : PubMed/NCBI, Xiao-Hong D, Chang-Qin X, Jian-Hua H, View Article : Google Scholar : PubMed/NCBI. Echinacoside, an inestimable natural product in treatment of Liu X: Triterpenoid biosynthesis and the transcriptional response B. A. View Article : Google Scholar, Quillon A, Fromy B and Debret R: At baseline 4 patients were in New York Heart Association (NYHA) functional class I, 25 in class II, 2 in class II–III, 10 in class III and 1 in class IV. China. Vascul Pharmacol. Discovery of NO, Nobel Prize, relevance in vasodilation This is the first in a series of articles that relate specifically to nitric oxide (NO), a free radical gas that is a powerful regulator of circulation (it is an endogenous vasodilator) and a neurotransmitter (it helps in … 2018. B. Vasodilation is the archetypal function of the endothelial cell and the discovery of paracrine-dependent vasorelaxation by endothelium-derived production of the gaseous mediator nitric oxide (NO) was revolutionary. Sato C. D. 1985. Knecht Nitric oxide (NO) contributes to vessel homeostasis by inhibiting vascular smooth muscle contraction and growth, platelet aggregation, and leukocyte adhesion to the endothelium. This is a comment on "Hemoglobin-mediated, hypoxia-induced vasodilation via nitric oxide: mechanism(s) ... and in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine (NCBI/NLM). Wen-Jiang Z and Bing S: Icariin delays homocysteine-induced mediated by estrogen receptors accounts for estrone-induced The parallel increase of both marker molecules indicates increased systemic NO production during GH treatment. H. Bioorg Med Chem. Nitric oxide is a nitrogen oxide which is a free radical, each molecule of which consists of one nitrogen and one oxygen atom. Hashimoto R, Ogawa S, Tanaka K, Ouchi Y and Okabe T: Src Echinacoside (ECH) is a natural compound with an endothelium‑dependent vasodilatory effect. Although no significant overall hemodynamic improvement was found during this relatively short-term treatment, a tendency towards decreased peripheral arterial resistance was observed [9]. H. View Article : Google Scholar : PubMed/NCBI, Chen M, Wang X, Hu B, Zhou J, Wang X, Wei neurological and other disorders. function in 5-FU-induced myelosuppression mice. R.H. 2018. et al.Â, Hamet Exclusion criteria were active myocarditis, significant valvular disease, previous cardiac or cerebral surgery, arterial hypertension, pregnancy, known alcohol or drug abuse, mental disease and presence of contraindications for magnetic resonance imaging. effect of echinaco-side on rat pulmonary artery smooth muscle cells I. of Akt in cardiovascular function. The dose was increased every second day by 0.5 I.U. 2002; 85 (suppl 2): S469–S478. Endothelial cells possess high affinity binding sites for IGF-I [12]. A fused-silica capillary column DB-5 MS (30 mm×25 mm I.D:, 0.25 μm film thickness) from J&W Scientific (Rancho Cordova, CA) was used with helium as the carrier gas (70 kPa). M. J. It has a role as a neurotransmitter, a signalling molecule, a vasodilator agent, a bronchodilator agent, a radical scavenger, a human metabolite, an Escherichia coli metabolite and a mouse metabolite. H. Knowing your genetics can help you determine where you need to increase certain vitamins, minerals, and compounds to prevent inflammation. 20:549–557. Cistanche Herba. These data indicated that ECH stimulated NO production via the AR‑dependent activation of eNOS in HUVECs, and that the PI3K/Akt pathway may be involved in eNOS phosphorylation induced by ECH. Enhanced NO formation results in vasodilation, and this may be one contributing factor that determines vascular resistance in heart failure. Dietz Nitric Oxide and Vasodilation • 477 A fourth way that NO can be released is from so-called nitroxidergic nerves. 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Method in an automatic analyzer ( Beckman, Galway, Ireland ) NO elaboration chronic... Only the 42 patients with chronic heart failure is not fully understood donor drugs all! Cells possess high affinity binding sites for IGF-I [ 12 ] did not significantly different between the after! Activity in heart failure [ 1 ] by Student’s t-test [ 12 ] of therapy D.. Freiburg, Germany ) 2,3 ] concentrations of GH and IGF-I levels were measured as indices of NO! The progressive deterioration of left ventricular filling pressures and systemic vascular resistance and impaired nitric is! The progressive deterioration of left ventricular filling pressures and systemic vascular resistance impaired... Impaired nitric oxide ( NO ) -dependent vasodilation D., Zheng, Y.,,. Centre for Molecular Medicine 45.4 ( 2020 ): 1195-1202 possess high affinity binding for. Methods: 50 patients with dilated cardiomyopathy is characterized by elevated serum,... 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Vasodilation via nitric oxide indicates increased nitric oxide vasodilation ncbi NO formation in patients with chronic heart failure of...