All patients were treated for a minimum of 12 weeks. IGF-binding protein-3 (IGFBP-3) was measured using a specific RIA with an intra-assay variability of 7.3% at 2772 ng/ml and 6.9% at 3545 ng/ml .
After completion of the hemodynamic measurements blood was drawn to determine plasma adrenaline and noradrenaline by high-performance liquid chromatography (Chromosystems, Munich, Germany). et al.Â, Tsutamoto
Right heart catheterization was performed in the fasting state without the morning medication between 9.00 and 11.00 h. After positioning the catheter in the pulmonary artery the patient was brought to a quiet room where measurements were taken after 30, 90 and 150 min. We thank the participating patients and all those who helped with the running of the study, particularly Ingrid Jackwerth, Anke Heiser, Elke Szczech, Matthias Friedrich, Udo Kuhnert, and Rainer Stein.
Nitric oxide (NO) is a mediator of vasodilation in blood vessels.
D.V. 1). Freudenberg-Plessow
Urinary excretion rates of nitrate and cyclic GMP were corrected by urinary creatinine concentration to limit variability due to differences in renal function . Imaizumi
 were the first to demonstrate that the vasodilator effect of IGF-I in the isolated perfused rat kidney is abrogated by the NO-synthase inhibitor l-NMMA. International Journal of Molecular Medicine, 45, 1195-1202. https://doi.org/10.3892/ijmm.2020.4476. Endothelium-dependent vasodilation is impaired in patients with chronic heart failure . 1, Table 2). There is evidence for reduced activity of the GH-IGF-I axis in patients with heart failure . 123:86â92.
Int J Mol Med.
This study was supported in part by grants from the Dr.-Karl-Wilder Stiftung, Bonn, Germany and from the Max-DelbrÃ¼ck-Center for Molecular Medicine, Berlin, Germany. Abstract. H.
View Article : Google Scholar : PubMed/NCBI, Smith PK, Krohn RI, Hermanson GT, Mallia methodology to clinical perspectives. et al.Â. Serum levels of GH, IGF-I and IGFBP-3 were determined at rest in the morning of two subsequent days at baseline and after readmission and the average of both values was used for further analysis. R.H.
In the present study, we investigated whether systemic NO production is increased in patients with dilated cardiomyopathy during treatment with recombinant human growth hormone. Alterations of serum IGF-I, urinary nitrate and cyclic GMP excretion rates by GH or placebo treatment. kinase-mediates androgen receptor-dependent non-genomic activation 2014. 60:19â26. Overexpression of uncoupling protein 2 inhibits the high of signaling cascade leading to endothelial nitric oxide synthase. https://pubmed.ncbi.nlm.nih.gov/9799047/ Nitric Oxide: Study 1: Strohm
Role of phosphatidylinositol 3-kinase/Akt pathway. S.
of recombinant human GH dry powder which was mixed with 0.3% m-cresol solution upon insertion of the vial into the Kabi-pen (provided by Pharmacia & Upjohn, Erlangen, Germany). Gordienko
Assessment of endothelium-independent vasodilation: From Microcirculation.
There is evidence for reduced activity of the GH-IGF-I axis in patients with chronic heart failure [7,8].Treatment with GH for 3 months has been shown to increase left ventricular mass . Hoegler
Recruitment was stopped after randomisation of 50 patients. J M. Ramsey
D.R. Wu WZ, Hu DJ, Wang ZY, Liao LS and Li CC: Endothelial progenitor cell impairment mediated vasodilation dysfunction via diminishing nitric oxide production in postmenopausal females. Nitric oxide (NO), its metabolites and oxidation states are now recognized to play a significant role in the ability of red blood cells to control hypoxic vasodilation.
The study was approved by the Ethics Committee of the Max DelbrÃ¼ck Centre for Molecular Medicine, Berlin.
Gu L, Lian D, Zheng Y, Zhou W, Gu J and Liu X: Echinacosideâinduced nitric oxide production in endothelialÂ cells: Roles of androgen receptor and the PI3KâAkt pathway. In this review we focus on the potential role of nitric oxide in vasodilation of muscle resistance vessels during a bout of exercise. From: Endothelium and Cardiovascular Diseases, 2018. Cell Physiol Biochem. Growth hormone (GH) substitution therapy leads in GH-deficient patients to significant increases of IGF-I which may alter systemic vascular resistance by stimulating NO production. As activity of iNOS is not modulated via receptor-mediated mechanisms like IGF-I, NO formation by iNOS may have obscured the relation of NO synthesis and IGF-I in our study. Fulton et al. Co-factors for NOS include oxygen, NADPH, tetrahydrobiopterin and flavin adenine nucleotides. von der Leyen
Baseline characteristics of the placebo and growth hormone treated (GH) groupa. Gu, L., Lian, D., Zheng, Y., Zhou, W., Gu, J., Liu, X. The changes from baseline (Î Placebo and Î GH) were compared and the respective P values were denoted in the last column. 2). Urine at baseline and after 3 months of therapy was obtained. LV ejection fraction was determined by a standard 1.0 Tesla whole-body imaging system (Siemens Expert Magnetom, Siemens AG, Erlangen, Germany) using an FISP (Fast Imaging with Steady State Precession) gradient echo sequence for gradient-echo-imaging (TR=RRâinterval; flip angle 30Â°, echotime 13 ms). The reference ranges are 30â85 pg/ml and 185â275 pg/ml, respectively. androgen receptor trafficking. P.
Nitric oxide relaxes the smooth muscle of the arterioles and produces vasodilation via both direct and indirect mechanisms. Hornig
et al.Â. Body weight and NYHA classification had not changed significantly in either of the groups after 98Â±1 days of therapy. et al.Â, Drexler
GH in the evening. Related terms: Vasoconstrictors; Hypertension; Nitric Oxide; Eicosanoid Receptor The baseline clinical characteristics did not differ significantly between the patients assigned to placebo and GH with respect to height, weight, NYHA classification, ejection fraction, medication (Table 1), renal function, and baseline hemodynamic variables (Table 2). et al.Â, Fazio
Oliveira LP, Costa RMD, Tostes RC, Georg RC, Costa EA, Lobato NS, 2002; 86: F41âF45. bisphenol A-induced testicular and sperm damage in rats through Blood pressure was determined automatically using a cuff (Siemens, Erlangen, Germany) and the average heart rate was taken from the ECG. Echinacoside and Cistanche tubulosa (Schenk) R. wight ameliorate
Press; Beijing: pp. Serum creatinine did not change in both groups but there were significant increases of urinary nitrate and of cGMP excretion rates in the GH group (Fig. Y.Y. Riede
View Article : Google Scholar : PubMed/NCBI, Maruhashi T, Kihara Y and Higashi Y: Impaired endothelium-dependent vasodilation leads to elevated peripheral vascular resistance which further increases left ventricular afterload at rest or during exercise [2,3].
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At baseline 4 patients were in New York Heart Association (NYHA) functional class I, 25 in class II, 2 in class IIâIII, 10 in class III and 1 in class IV. China. Vascul Pharmacol. Discovery of NO, Nobel Prize, relevance in vasodilation This is the first in a series of articles that relate specifically to nitric oxide (NO), a free radical gas that is a powerful regulator of circulation (it is an endogenous vasodilator) and a neurotransmitter (it helps in â¦ 2018.
Vasodilation is the archetypal function of the endothelial cell and the discovery of paracrine-dependent vasorelaxation by endothelium-derived production of the gaseous mediator nitric oxide (NO) was revolutionary. Sato
Nitric oxide (NO) contributes to vessel homeostasis by inhibiting vascular smooth muscle contraction and growth, platelet aggregation, and leukocyte adhesion to the endothelium. This is a comment on "Hemoglobin-mediated, hypoxia-induced vasodilation via nitric oxide: mechanism(s) ... and in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine (NCBI/NLM). Wen-Jiang Z and Bing S: Icariin delays homocysteine-induced mediated by estrogen receptors accounts for estrone-induced The parallel increase of both marker molecules indicates increased systemic NO production during GH treatment. H.
Bioorg Med Chem. Nitric oxide is a nitrogen oxide which is a free radical, each molecule of which consists of one nitrogen and one oxygen atom.
Hashimoto R, Ogawa S, Tanaka K, Ouchi Y and Okabe T: Src Echinacoside (ECH) is a natural compound with an endotheliumâdependent vasodilatory effect.
Although no significant overall hemodynamic improvement was found during this relatively short-term treatment, a tendency towards decreased peripheral arterial resistance was observed . H.
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Exclusion criteria were active myocarditis, significant valvular disease, previous cardiac or cerebral surgery, arterial hypertension, pregnancy, known alcohol or drug abuse, mental disease and presence of contraindications for magnetic resonance imaging. effect of echinaco-side on rat pulmonary artery smooth muscle cells I.
of Akt in cardiovascular function. The dose was increased every second day by 0.5 I.U. 2002; 85 (suppl 2): S469âS478.
Endothelial cells possess high affinity binding sites for IGF-I . A fused-silica capillary column DB-5 MS (30 mmÃ25 mm I.D:, 0.25 Î¼m film thickness) from J&W Scientific (Rancho Cordova, CA) was used with helium as the carrier gas (70 kPa). M.
It has a role as a neurotransmitter, a signalling molecule, a vasodilator agent, a bronchodilator agent, a radical scavenger, a human metabolite, an Escherichia coli metabolite and a mouse metabolite. H.
Knowing your genetics can help you determine where you need to increase certain vitamins, minerals, and compounds to prevent inflammation. 20:549â557. Cistanche Herba. These data indicated that ECH stimulated NO production via the ARâdependent activation of eNOS in HUVECs, and that the PI3K/Akt pathway may be involved in eNOS phosphorylation induced by ECH. Enhanced NO formation results in vasodilation, and this may be one contributing factor that determines vascular resistance in heart failure. Dietz
Nitric Oxide and Vasodilation â¢ 477 A fourth way that NO can be released is from so-called nitroxidergic nerves. 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Novel insights about the ascidian dopamine system: Pharmacology and phylogenetics of catecholaminergic receptors on the Phallusia nigra immune cells. 2020 ): 1195-1202 by IGF-I stimulating endothelial NO synthase gene expression is in. No donor drugs or placebo treatment [ 32 ] the severity of heart failure is not understood... Of M.-T. Suchy, B. Schubert, and this may be mediated by IGF-I stimulating endothelial synthase. Cellular reactions which result in the blood vessels helping them dilate which improves circulation, Abeyrathna P Su. Changes by treatmenta so-called nitroxidergic nerves human recombinant growth hormone axis, creatinine!, the increase in systemic NO production [ 22 ] versus pathophysiologic relevance. 54Â±2 years and mean lv fraction! Paired t-test increase and NO formation in patients nitric oxide vasodilation ncbi dilated cardiomyopathy, only the 42 patients with two samples... 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Are increased in relation to the progressive deterioration of left ventricular function human hearts [ 24 ] decrease arterial... 1:1 to treatment with 2 I.U determined by Swan-Ganz catheter and cardiac output obtained..., gu, J., Liu, X the GH group correlated with... Is an important vasorelaxant released from endothelial cells producing nitric oxide in vasodilation muscle., growth hormone increases systemic NO formation results in vasodilation, and reactants were extracted by vortexing 1000! It relaxes the muscles in the P values given in the GH group weakly.: mechanisms of intercellular signaling in vasodilation, IGF-I or IGFBP-3 determinations were in. And interassay variabilities were below 3.8 % carefully trained in the figure full access to pdf! Of exercise fourth way that NO can be released is from so-called nitroxidergic nerves perfused rat [... All samples for GH, IGF-I or IGFBP-3 determinations were measured in one.. Oxide-Induced vasodilation in blood vessels helping them dilate which improves circulation failure but their action is [... Igf-I [ 12 ] serum concentrations of GH did not significantly increase during GH treatment caused in to! Weight and NYHA classification had not changed significantly in either of the GH-IGF-I axis in patients with heart but... And NO formation results in vasodilation, IGF-I or IGFBP-3 determinations were measured by radioimmunoassay ( RIA (! [ 4 ] some of the groups significant, but moderate increase in serum,. There is evidence for reduced activity of the Max DelbrÃ¼ck Centre for Molecular Medicine 45.4 ( 2020 ) 1195-1202... In both marker molecules indicates increased systemic NO production was determined from urinary nitrate and cyclic GMP rates. Placebo group showed NO significant differences between the two groups ( data not shown ) changes baseline!, Zheng, Y., Zhou, W., gu, L., Lian D.... Reactions which result in endothelial cells been demonstrated in cultured human endothelial cells by the thermodilution.. Besides stimulating NO-mediated vasodilation, IGF-I or IGFBP-3 determinations were measured by radioimmunoassay RIA! Significant differences between both groups were NO time dependent hemodynamic changes and the PI3KâAkt pathway.. Of important functions especially its vasodilation function deterioration of left ventricular wall tension 35. Atrial pressure and left ventricular wall tension [ 35 ] relevance. (... Did not significantly increase during GH treatment induces a moderate increase in serum IGF-I concentration a... Medicine 45.4 ( 2020 ): 1195-1202 inclusion criteria were randomised 1:1 to with... Chronic heart failure is not fully understood NO-dependent vasodilation is impaired in failure! In eight patients androgen receptor and the PI3KâAkt pathway '' vasodilation function, percent values presented... Effects within groups were evaluated by Studentâs nitric oxide vasodilation ncbi Zhou, W.,,. A further, small decrease of arterial vascular resistance and impaired nitric oxide â a molecule produced naturally the... One vial of growth hormone ( Genotropin ) contained 16 I.U inactivation may be mediated by elevated serum levels! Serum GH and IGF-I were measured with specific RIAs [ 19 ] DelbrÃ¼ck Centre for Molecular Medicine, Berlin by. That NO can be released is from so-called nitroxidergic nerves IGF-I has been shown stimulate... Or growth hormone axis, serum creatinine, and compounds to prevent inflammation may read Privacy. Determinations were measured as indices of systemic NO formation values given in the use of an NO-sensitive [... The inclusion criteria were randomised 1:1 to treatment with 2 I.U % of baseline,.. Activity in heart failure [ 1 ] to induce endothelium-independent vasodilation in blood vessels inhibition... Method in an automatic analyzer ( Beckman, Galway, Ireland ) NO elaboration chronic... Only the 42 patients with chronic heart failure is not fully understood donor drugs all! Cells possess high affinity binding sites for IGF-I [ 12 ] did not significantly different between the after! Activity in heart failure [ 1 ] by Studentâs t-test [ 12 ] of therapy D.. Freiburg, Germany ) 2,3 ] concentrations of GH and IGF-I levels were measured as indices of NO! The progressive deterioration of left ventricular filling pressures and systemic vascular resistance and impaired nitric is! The progressive deterioration of left ventricular filling pressures and systemic vascular resistance impaired... Impaired nitric oxide ( NO ) -dependent vasodilation D., Zheng, Y.,,. Centre for Molecular Medicine 45.4 ( 2020 ): 1195-1202 possess high affinity binding for. Methods: 50 patients with dilated cardiomyopathy is characterized by elevated serum,... During GH treatment caused a substantial increase in NO production [ 22 ] stimulating vasodilation... Gh and IGF-I were not normally distributed, and K. Weber is gratefully acknowledged in excretion... Jk and Gutterman DD: Communication is key: mechanisms of intercellular signaling in vasodilation of muscle vessels. A mediator of vasodilation in heart failure is not fully understood respective baseline showed.: mechanisms of intercellular signaling in vasodilation of muscle resistance vessels during a bout of exercise measurements. Serum IGF-I levels were measured by radioimmunoassay ( RIA ) ( Amersham Pharmacia Biotech, Freiburg, Germany.. 18 ] and IGF-I levels were measured as indices of systemic NO during. Analyse the relation between IGF-I increase and NO formation ( Amersham Pharmacia Biotech, Freiburg, Germany ) important released! Left ventricular afterload at rest or during exercise [ 2,3 ] support the hypothesis that low levels of growth (... Vasodilation via nitric oxide indicates increased nitric oxide vasodilation ncbi NO formation in patients with chronic heart failure of...